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Test Category: Immunology

Investigation Name:HLA B27

Alias or Abbreviation:HLA B27

Intro:

Indication: Aid in the diagnosis of patients who present with back pain that may be rheumatoid or ankylosing spondylitis in origin. Further information: The Human Leukocyte Antigen (HLA) B27 is an MHC class 1 molecule whose presence has a strong association with ankylosing spondylitis as well as other disorders including Reiter’s syndrome, psoriatic arthritis and arthropathies associated with inflammatory bowel disease. This assay tests for the presence of HLA-B27 to aid in the diagnosis of patients who present with back pain that may be rheumatoid or ankylosing spondylitis in origin. Indeterminate results are referred for HLA B27 by PCR

Requestable Seperately?Yes

Minimum Sample Volume:1mL

Expected Turnaround Time:Frequency of testing: Daily Target: 2 days

Test Code:B27

Sample Type:

EDTA

Complex Reference Range:Positive/ Negative/ Indeterminate

Collection Conditions / Other Information:1st class post or Transport

Referred to Another Laboratory?No

Storage Requirements:Ambient temperature

NPEX / PDF Reporting Available:Yes

EQA Scheme:Yes

Further information:Laboratory use only: Do not refrigerate. Must be processed within 4 days of venepuncture.

Lead Contact Details:rwh-tr.bcpsimmunologyclinicalqueries@nhs.net

Email Address For Chasing Results:rwh-tr.ImmunologyLab@nhs.net

Methodology:Flow Cytometry

UKAS Reference Number:Awaiting assessment

Site Sample Tested:Immunology, New Cross Hospital, Wolverhampton