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Test Category: Immunology

Investigation Name:Extractable Nuclear Antigen (ENA Screen)

Alias or Abbreviation:ENA, Ro, La, Sm, RNP, Jo-1, Scl-70

Intro:

Indication: Connective Tissue Disease (CTD) Further information: The ENA screen includes the following antigens: • SS-A/Ro (60 kDa, 52 kDa) • SS-B/La • SmD3 peptide • U1RNP (RNP 70, A, C) • Scl-70, • Jo-1 proteins • Centromere B.

Requestable Seperately?Yes

Minimum Sample Volume:1mL

Expected Turnaround Time:Frequency of testing: Daily Target: 7 days

Test Code:ENA

Sample Type:

Serum Gel Tube (Gold top)/ Plasma

Complex Reference Range:Positive/ Equivocal/ Negative

Collection Conditions / Other Information:1st class post or Transport

Referred to Another Laboratory?No

Storage Requirements:Ambient temperature

NPEX / PDF Reporting Available:Yes

EQA Scheme:Yes

Lead Contact Details:rwh-tr.bcpsimmunologyclinicalqueries@nhs.net

Email Address For Chasing Results:rwh-tr.ImmunologyLab@nhs.net

Methodology:Fluoroenzymeimmunoassay

UKAS Reference Number:9580 (recommended)

Site Sample Tested:Immunology, New Cross Hospital, Wolverhampton

Minimum Request Interval (MRI):180 days (6 months) over 16y only