Epidermal antibodies Screen (Epidermal Intercellular antibodies, Epidermal Basement antibodies)

Investigation Name:Epidermal antibodies Screen (Epidermal Intercellular antibodies, Epidermal Basement antibodies)

Alias or Abbreviation:Skin, Pemphigus, Pemphigoid

Intro:

Indication: Autoimmune skin diseases Further information: The blistering skin diseases where IIF is used are: Pemphigus Vulgaris (PV) Serum antibodies directed against the cell surface of epidermal keratinocytes are found in 90% of patients with Pemphigus and correlate with disease activity. Bullous Pemphigoid Serum antibodies directed against the basement membrane are present in 70% of patients. Antibody levels do not reflect disease activity and antibody tends to persist even following successful therapy. Antibodies are uncommon in normal individuals or in other skin diseases.

Requestable Seperately?Yes

Minimum Sample Volume:1mL

Test Code:EPID

Sample Type:

Serum Gel Tube (Gold top)

Complex Reference Range:Positive/ Negative

Collection Conditions / Other Information:1st class post or Transport

Referred to Another Laboratory?No

Storage Requirements:Ambient temperature

NPEX / PDF Reporting Available:Yes

EQA Scheme:Yes

Lead Contact Details:rwh-tr.bcpsimmunologyclinicalqueries@nhs.net

Email Address For Chasing Results:rwh-tr.ImmunologyLab@nhs.net

Methodology:Indirect Immunofluorescence

UKAS Reference Number:Awaiting assessment

Site Sample Tested:Immunology, New Cross Hospital, Wolverhampton

Minimum Request Interval (MRI):180 days (6 months) over 16y only